Perform Raman normalization of EEMs

Normalizes the fluorescence intensity of EEMs to Raman Units (R.U.) based on the area of the water Raman peak (Lawaetz and Stedmon, 2009).

Usage

raman_normalize(eemlist)

Source

Lawaetz, A. J., & Stedmon, C. A. (2009). Fluorescence Intensity Calibration Using the Raman Scatter Peak of Water. Applied Spectroscopy, 63(8), 936-940. doi:10.1366/000370209788964548

Arguments

eemlist

An eemlist object.

Value

An eemlist object with EEMs normalized to Raman Units.

Details

The function uses the Raman peak area provided in the metadata to normalize the EEMs. Therefore, metadata must be added to the samples prior to normalization using add_metadata().

Examples

eemlist <- add_metadata(metadata, example_eems)
correct_eem <- raman_normalize(eemlist)